Protection of Guinea Pigs against Leptospira interrogans Serovar Lai by LipL21 DNA Vaccine
نویسندگان
چکیده
منابع مشابه
Classification of Leptospira interrogans serovar lai strain 017 by using monoclonal antibodies.
Leptospira interrogans serovar lai was identified in China in 1966 as a new serovar of the icterohaemorrhagiae serogroup by cross-absorption tests. In this study, we established three hybridoma cell lines producing monoclonal antibodies (MAbs) of the immunoglobulin G3 subclass (LW1, LW2, and LW3) and of the immunoglobulin M class (LW4a) against serovar lai strain 017 by the cell fusion techniqu...
متن کاملImmunological characteristics of the glycolipid antigen of Leptospira interrogans serovar lai.
The protective antigen (PAg), a glycolipid substance, was extracted from Leptospira interrogans serovar lai strain 017 with a chloroform-methanol-water (1:2:0.8 [vol/vol/vol]) solution and partially purified by silica gel column chromatography. The PAg was not detected by Coomassie brilliant blue staining in sodium dodecyl sulfate-polyacrylamide gel electrophoresis but was observed as a smearli...
متن کاملRe-characterization of an extrachromosomal circular plasmid in the pathogenic Leptospira interrogans serovar Lai strain 56601.
In China, Leptospira interrogans serovar Lai strain 56601 (str.56601) is one of main pathogenic strains that cause severe leptospirosis in both human and animals. The genome of this organism was completely sequenced in 2003. However, in 2011, we identified and corrected some assembly errors in the str.56601 genome due to the repeat sequences widely distributed in the Leptospira genome. In this ...
متن کاملA genomic island of the pathogen Leptospira interrogans serovar Lai can excise from its chromosome.
An examination of the two Leptospira interrogans genomes sequenced so far reveals few genetic differences, including an extra DNA region, 54 kb in length, in L. interrogans serovar Lai. This locus contains 103 predicted coding sequences that are absent from the genome of L. interrogans serovar Copenhageni, of which only 20% had significant BLASTP hits in GenBank. By analyzing the L. interrogans...
متن کاملExpression and characterization of an iron-regulated hemin-binding protein, HbpA, from Leptospira interrogans serovar Lai.
In an earlier study, based on the ferric enterobactin receptor FepA of Escherichia coli, we identified and modeled a TonB-dependent outer membrane receptor protein (LB191) from the genome of Leptospira interrogans serovar Lai. Based on in silico analysis, we hypothesized that this protein was an iron-dependent hemin-binding protein. In this study, we provide experimental evidence to prove that ...
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ژورنال
عنوان ژورنال: Cellular & Molecular Immunology
سال: 2008
ISSN: 1672-7681,2042-0226
DOI: 10.1038/cmi.2008.48